the Extraction and identification of flavonoids of the Prosopis farcta (L.) leaf extract and study of the anti-inflammatory and protective effect on DNA damage. Extraction and identification of flavonoids of the Prosopis farcta (L.) leaf extract and study of the anti-inflammatory and protective effect on DNA damage.

Abstract

Abstract
Background: Plant extracts have been used as therapeutic substances and have gained importance for
human health around the world. The current study aims to identify flavonoids in the leaves of Prosopis farcta
and to verify the anti-inflammatory efficacy of the leaf methanol extract, in addition to its ability to protect
radiation-induced DNA damage in the presence of H2O2.
Methods: The reflux method was applied to extract the flavonoids in leaves of P. farcta. The type of
flavonoids present in the methanol extract was fixed by thin-layer chromatography (TLC) and highperformance-liquid chromatography (HPLC) depending on the reference markers, the role of the extract as an
anti-inflammatory substance was studied using the denaturation method of bovine serum albumin. The
activity of different concentrations (250, 500 and 1000) micrograms/ milliliter, of P. farcta extract in
protecting DNA damage induced by radiation with hydrogen peroxide and ultraviolet radiation was
determined laboratory. Results: The TLC and calibration plot of standard luteolin, hesperidin and rutin
showed that the alcoholic extract of P. farcta was found to contain luteolin, hesperidin and rutin, with the
retardation factor 0.83, 0.4, 0.56 respectively. Methanolic extract of leaves at 500 μg/ml had antiinflammatory action similar to that standard medicine aspirin. The P. farcta extract possesses protective
properties against DNA oxidative stress at concentration of 1000 μg/ml.
Conclusion: Three flavonoids were found in refluxed P. farcta leaves with 80% methanol and it can be used as
a potential source of natural anti-inflammatory and DNA damage protecting.